Technical Report
Glass needle-based chromosome microdissection—How to set up probes for molecular cytogenetics?
1 PhD, Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Am Klinikum 1, D-07747 Jena, Germany
Address correspondence to:
Thomas Liehr
Institut für Humangenetik, Am Klinikum 1, D-07747 Jena,
Germany
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Article ID: 100004VAM08AR2020
doi: 10.5348/100004VAM08AR2020TR
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Al-Rikabi A, Liehr LB, Liehr T. Glass-needle based chromosome microdissection—How to set up probes for molecular cytogenetics? Video J Clin Res 2020;2:100004VAM08AR2020.Abstract
Aims: Molecular cytogenetics is basically the use of fluorescence in situ hybridization (FISH). To perform FISH, among others species-specific DNA-probes are required. Glass needle-based chromosome microdissection is a feasible approach to establish corresponding probes. More specifically, by glass needle-based chromosome microdissection whole chromosome and partial chromosome painting probes can be established.
Methods: Glass needle-based chromosome microdissection is still much more successful and cost-effective for production of FISH probes, than comparable laser-based chromosome microdissection approaches. Thus, here we present the first video protocol how to practically perform this approach.
Results: Technical details on necessary solutions have been previously published elsewhere and are also referred to in the video, however, the practical manual work cannot be better described than done here, by a short movie.
Conclusion: Based on this video, it should be much easier now for other laboratories to establish this technology.
Keywords: Fluorescence in situ hybridization (FISH), Glass needle-based chromosome microdissection, Partial chromosome paint, Whole chromosome paint
Supporting Information
Author Contributions:
Ahmed Al-Rikabi - Acquisition of data, Revising it critically for important intellectual content, Final approval of the version to be published
Leon B Liehr - Revising it critically for important intellectual content, Final approval of the version to be published
Thomas Liehr - Substantial contributions to conception and design, Analysis of data, Interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Data Availability Statement:The corresponding author is the guarantor of submission.
Consent For Publication:Written informed consent was obtained from the patient for publication of this article.
Competing Interests:Authors declare no conflict of interest.
